A Comprehensive Guide to Selecting and Using Sterile Sample Bags: Specification Analysis and Operating Procedures

 

In microbiology experiments and laboratory liquid handling, sterile sample bags have become crucial tools due to their sealing properties, durability, and sterility. This article will in - depth analyze their core uses, common specifications, and standardized operating procedures to help users make efficient selections and use them correctly.

 

I. Core Functions and Application Scenarios

Sterile sample bags are mainly used in the following scenarios:

1. Microbial sample processing: This includes sample collection, homogenization, and enrichment culture to ensure that samples are not contaminated during transfer.

2. Storage and transfer of liquid reagents: They are suitable for the short - term storage of laboratory liquid reagents, avoiding the risk of cross - contamination or leakage.

 

II. Common Specifications and Loading Recommendations

According to experimental requirements, the following four mainstream specifications can be selected:

1. Stand - up self - sealing homogenizing bag (20×32 cm): The flat - bottom design facilitates experimental operations. The recommended loading is 50 - 300 milliliters.

2. Full - width microporous filtration type (19×30 cm): It has a built - in microporous membrane and is suitable for sample processing requiring filtration. The recommended loading is 50 - 300 milliliters.

3. Side non - woven fabric filtration type (19×30 cm): The side non - woven fabric design improves homogenization efficiency. The recommended loading is 50 - 300 milliliters.

4. Flat - mouth sterile homogenizing bag (19×30 cm): It has a universal design and is compatible with various experimental equipment. The recommended loading is 50 - 300 milliliters.

 

III. Operating Procedures and Key Points of Sterility Control

To ensure the accuracy of samples, the following steps should be strictly followed:

1. Preparation work

- Select a bag that matches the sample volume and check for any damage or contamination.

- Prepare sterilization tools (such as sterile tweezers and syringes) and protective equipment (masks and gloves).

2. Sampling operation

- Avoid touching the inner wall when tearing the sealing strip and directly transfer the sample using sterile tools.

- For liquid samples, control the loading to prevent overflow; for solid samples, disperse them evenly to reduce air bubbles.

3. Sealing and labeling

- Fold the bag mouth twice when sealing to ensure complete sealing.

- Label the sample type, collection time, and operator information to avoid confusion.

 

IV. Storage and Environmental Protection Precautions

1. Storage conditions: Store in a cool and dry environment, avoiding high temperatures or direct sunlight to prevent material aging.

2. Use period: Pay attention to the expiration date marked on the packaging. Using expired products may affect the sterility performance.

3. Environmental protection requirements: Prioritize the use of recyclable low - density polyethylene materials to reduce the generation of plastic waste.

 

V. Purchase Recommendations

1. Match experimental requirements: If homogenization is required, give priority to models with filtration functions; for regular liquid storage, choose the flat - mouth design.

2. Capacity compatibility: Avoid exceeding the recommended maximum loading, as it may affect the sealing performance or homogenization effect.